Composite

Part:BBa_K2088016:Design

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-09)


nativeRBS-pbaA1A2B with a double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 116
    Illegal NgoMIV site found at 776
    Illegal NgoMIV site found at 1383
    Illegal NgoMIV site found at 1407
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An optimization of BBa_K2088669 with a designed rbs.


Source

This part is an optimization of BBa_K2088669.

References

Wang C, Chen Q, Wang R, Shi C, Yan X, He J, et al. A Novel Angular Dioxygenase Gene Cluster Encoding 3-Phenoxybenzoate 1′,2′-Dioxygenase in Sphingobium wenxiniae JZ-1. Applied & Environmental Microbiology 2014;80:3811-8.